{"text_format":"2","workbook":"xlsx","n_sheets":7,"sheets":["1.Plate_layout_&HiFimix","2.Amplify_DNA_targets","3. Partially digest amplicons","4. Ligate indexes","5. Clean-up Library","6. Amplify Library","7. Second Cleanup"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"1.Plate_layout_&HiFimix","index":0,"n_rows":23,"n_cols":13,"rows_kept":23,"cut":false,"hidden":false,"merged":[[1,1,1,2],[2,1,2,2],[3,1,3,2],[17,0,17,4],[18,0,18,4],[20,0,20,4],[21,0,21,4],[22,0,22,4],[19,0,19,4]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date:"]
[]
["PREPARE DNA PLATE"]
["DNA plate layout",1,2,3,4,5,6,7,8,9,10,11,12]
["A","A1",{"$nulls":10},"A12"]
["B",null,"B2"]
["C"]
["D"]
["E",{"$nulls":5},"E6"]
["F"]
["G",{"$nulls":11},"G12"]
["H","H1",{"$nulls":10},"H12"]
[]
[]
["Procedures",{"$nulls":4},"volume (µl )"]
["1. DNA (1-100 ng) ",{"$nulls":4},7.5]
["OPTIONAL: safe stopping point, store at  4⁰C overnight or -15⁰C longer "]
["2. Add 5X AmpliSeq HIFI Mix (red cap)",{"$nulls":4},5]
["3. Briefly centrifuge"]
["4. Mix and transfer 2 X 5 µl to 2 new plates (pool 1 & pool 2)",{"$nulls":4},"2 X 5 µl"]

{"sheet":"2.Amplify_DNA_targets","index":1,"n_rows":35,"n_cols":13,"rows_kept":35,"cut":false,"hidden":false,"merged":[[31,0,31,8],[32,0,32,8],[33,0,33,8],[34,0,34,8],[0,1,0,2],[1,1,1,2],[2,1,2,2],[3,1,3,2],[30,10,30,12],[27,0,27,8],[28,0,28,8],[29,0,29,8],[30,0,30,8]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date:"]
[]
["AMPLIFY DNA TARGETS"]
["Primer Pool 1",1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B8","v":"A1"},{"$f":"'1.Plate_layout_&HiFimix'!C8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M8","v":"A12"}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
[]
["Primer Pool 2",1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B8","v":"A1"},{"$f":"'1.Plate_layout_&HiFimix'!C8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M8","v":"A12"}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
[]
[]
["Procedures",{"$nulls":8},"volume (µl )"]
["1. Add 5µL 2xAmpliSeq Custom DNA Pool 1 (red cap) to each well of Pool 1 using an automatic pipet",{"$nulls":8},5]
["2. Add 5µL 2xAmpliSeq Custom DNA Pool 2 (blue cap) to each well of Pool 2 using an automatic pipet",{"$nulls":8},5]
["3. Close using lids or seal",{"$nulls":9},"AMP_DNA program"]
["4. Briefly centrifuge to collect all liquid at the bottom well, check to remove bubbles",{"$nulls":9},"99°C","2min","1X"]
["5. Place on a thermocycler ",{"$nulls":9},"99°C","15sec","21x"]
["6. Run the AMP_DNA Program",{"$nulls":9},"60°C","8min"]
["SAFE STOPPING POINT (keep at ≤10⁰C overnight or store at -15⁰C)",{"$nulls":9},"10°C","Pause"]

{"sheet":"3. Partially digest amplicons","index":2,"n_rows":25,"n_cols":13,"rows_kept":25,"cut":false,"hidden":false,"merged":[[17,0,17,8],[20,11,20,12],[24,0,24,8],[18,0,18,8],[19,0,19,8],[20,0,20,8],[21,0,21,8],[22,0,22,8],[23,0,23,8]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date"]
[]
["PARTIALLY DIGEST AMPLICONS"]
["Pool 1 + Pool 2",1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B8","v":"A1"},{"$f":"'1.Plate_layout_&HiFimix'!C8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L8","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M8","v":"A12"}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
[]
[]
["Procedures",{"$nulls":8},"volume (µl )"]
["1. Briefly centrifuge both plates with amplified DNA of primer pool 1 and primer pool 2"]
["2. Combine the 2 POOL plates in 1 plate using multichannel",{"$nulls":8},"2x10µL"]
["3. Aliquot 30 µL of FuPa (brown cap) in each well of 8 well PCR strip",{"$nulls":8},"8x30µL",null,"FUPA program"]
["4. Transfer 2µL FuPa reagent from the 8w-strip to each well of the DNA using multichannel pipette",{"$nulls":8},"2 µL",null,"50°C ","10min"]
["5. Seal the plate, vortex briefly and briefly centrifuge ",{"$nulls":10},"55°C","10min"]
["6. Run the FUPA program on the Thermocycler",{"$nulls":10},"62°C","20min"]
["SAFE STOPPING POINT (store at -15⁰C)",{"$nulls":10},"10°C","Pause (max 1 hr)"]

{"sheet":"4. Ligate indexes","index":3,"n_rows":28,"n_cols":13,"rows_kept":28,"cut":false,"hidden":false,"merged":[[26,0,26,9],[27,0,27,9],[23,11,23,12],[17,0,17,9],[18,0,18,9],[19,0,19,9],[20,0,20,9],[21,0,21,9],[22,0,22,9],[23,0,23,9],[24,0,24,9],[25,0,25,9]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date"]
[]
["LIGATE INDEXES"]
["Pool 1 + Pool 2 digested amplicons"]
[null,1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M16","v":0}]
[]
["Procedures",{"$nulls":9},"volume (µl )"]
["1. Briefly centrifuge the plate with Pool 1 + Pool 2 digested amplicons"]
["2. Remove the seal from the index plate: Index plate used:  A/B/C/D, expiry date: ___/_____/_____"]
["3. Add 4µL Switch solution(yellow cap) to each well (using multipette or using 50 µL aliquots in 8w strip and multichannel)",{"$nulls":9},4]
["4. Add 2µL Ampliseq CD Indexes from the corresponding well in the Indexes Plate",{"$nulls":9},2]
["5. Aliquot 30 µL of DNA Ligase (blue cap) in each well of 8 well PCR strip",{"$nulls":9},"8 x 30"]
["6. Transfer 2µL DNA Ligase from the 8w strip to each well of the DNA using multichannel pipette",{"$nulls":9},2,"LIGATE program"]
["!!! DO NOT change the order of the reagents",{"$nulls":10},"22°C ","30 min"]
["7. Seal the plate, vortex briefly and briefly centrifuge ",{"$nulls":10},"68°C","5 min"]
["8. Run the LIGATE program on the thermocycler",{"$nulls":10},"72°C","5 min"]
["SAFE STOPPING POINT (keep at ≤10⁰C overnight or store at -15⁰C)",{"$nulls":10},"10°C","Pause (up to 24hrs)"]

{"sheet":"5. Clean-up Library","index":4,"n_rows":32,"n_cols":13,"rows_kept":32,"cut":false,"hidden":false,"merged":[[31,0,31,9],[17,0,17,9],[18,0,18,9],[20,0,20,9],[27,0,27,9],[19,0,19,9],[21,0,21,9],[22,0,22,9],[23,0,23,9],[24,0,24,9],[25,0,25,9],[26,0,26,9],[28,0,28,9],[29,0,29,9],[30,0,30,9]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date"]
[]
["CLEAN-UP LIBRARY"]
["Library plate"]
[null,1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M16","v":0}]
[]
["Procedures",{"$nulls":9},"volume (µL/mL )"]
["1. Equilibrate AMPure XP Beads to RT for ≥30 min"]
["2. Freshly prepare 50 mL 70% ethanol (EtOH) solution (35 mL 100% EtOH + 15 mL PCR grade H2O)",{"$nulls":9},"50 mL"]
["3. Briefly centrifuge the plate with libraries"]
["4. Add 30 µl AMPure XP beads (vortex thoroughly before pipetting) to each well ",{"$nulls":9},"30 µL"]
["5. Seal the plate with an adhesive seal and vortex briefly till homogenous then briefly centrifuge"]
["6. Incubate at RT for 5 min"]
["7. Place on magnet and wait till liquid is clear ≥2 min"]
["8. Discard all supernatant with multichannel pipette",{"$nulls":9},"~60 µL"]
["9. WASH 2X: 1) Add 150 µL 70% EtOH, 2) incubate till clear (≥30sec), 3) discard supernatant",{"$nulls":9},"150 µL"]
["10. Briefly centrifuge the plate with beads"]
["11. Place on magnet (same orientation!) and wait till liquid is clear ≥30 sec"]
["12. Remove any residual EtOH with multichannel pipette and let air-dry for ≥ 10 min"]
["13. Inspect wells if all EtOH has evaporated, otherwise repeat step 12. "]
["IMMEDIATELY CONTINUE WITH AMPLIFY LIBRARY (NOT A SAFE STOPPING POINT)"]

{"sheet":"6. Amplify Library","index":5,"n_rows":27,"n_cols":14,"rows_kept":27,"cut":false,"hidden":false,"merged":[[17,0,17,9],[19,7,19,8],[20,7,20,8],[19,3,19,6],[20,3,20,6],[18,0,18,9],[26,0,26,9],[24,0,24,9],[25,0,25,9],[24,11,25,11],[21,0,21,9],[22,0,22,9],[23,0,23,9],[21,11,21,13]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date"]
[]
["AMPLIFY LIBRARY"]
["Cleaned up library plate"]
[null,1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M16","v":0}]
[]
["Procedures",{"$nulls":9},"volume (µl )"]
["1. Prepare the amplification master mix in a 15 mL falcon tube (for 96 reactions) "]
[null,null,null,"1X Lib Amp Mix (black cap)",null,null,null,"45 µL/reaction",null,"x100=",4500]
[null,null,null,"10X Library Amp Primers (pink cap)",null,null,null,"5 µL/reaction",null,"x100 =",500]
["2. Vortex amplification master mix",{"$nulls":10},"AMP_7 program"]
["3. Remove the 96w cleaned library plate (previous section) from the magnet. ",{"$nulls":10},"Cycles","Temp.","Time"]
["4. Add 50 µl amplification master mix to each well ",{"$nulls":9},50,1,"98⁰C","2 min"]
["5.  Seal the plate, vortex briefly and briefly centrifuge ",{"$nulls":10},7,"98⁰C","15 sec"]
["6. Run the AMP_7 program on the thermocycler",{"$nulls":11},"64⁰C","1 min"]
["SAFE STOPPING POINT (keep at ≤10⁰C overnight or store at -15⁰C)",{"$nulls":10},"Hold","10⁰C","up to 24 hrs"]

{"sheet":"7. Second Cleanup","index":6,"n_rows":42,"n_cols":13,"rows_kept":42,"cut":false,"hidden":false,"merged":[[17,0,17,9],[18,0,18,9],[19,0,19,9],[21,0,21,9],[22,0,22,9],[34,0,34,9],[37,0,37,9],[38,0,38,9],[20,0,20,9],[28,0,28,9],[29,0,29,9],[30,0,30,9],[31,0,31,9],[24,0,24,9],[25,0,25,9],[32,0,32,9],[33,0,33,9],[23,0,23,9],[39,0,39,9],[40,0,40,9],[36,0,36,9],[35,0,35,9],[41,0,41,9]]}
["Study identification:"]
["Executor:"]
["Experiment: "]
["Date"]
[]
["SECOND CLEAN-UP"]
["Amplified Library plate"]
[null,1,2,3,4,5,6,7,8,9,10,11,12]
["A",{"$f":"'1.Plate_layout_&HiFimix'!B9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C9","v":"B2"},{"$f":"'1.Plate_layout_&HiFimix'!D9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L9","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M9","v":0}]
["B",{"$f":"'1.Plate_layout_&HiFimix'!B10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L10","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M10","v":0}]
["C",{"$f":"'1.Plate_layout_&HiFimix'!B11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L11","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M11","v":0}]
["D",{"$f":"'1.Plate_layout_&HiFimix'!B12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G12","v":"E6"},{"$f":"'1.Plate_layout_&HiFimix'!H12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L12","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M12","v":0}]
["E",{"$f":"'1.Plate_layout_&HiFimix'!B13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L13","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M13","v":0}]
["F",{"$f":"'1.Plate_layout_&HiFimix'!B14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L14","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M14","v":"G12"}]
["G",{"$f":"'1.Plate_layout_&HiFimix'!B15","v":"H1"},{"$f":"'1.Plate_layout_&HiFimix'!C15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L15","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M15","v":"H12"}]
["H",{"$f":"'1.Plate_layout_&HiFimix'!B16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!C16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!D16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!E16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!F16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!G16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!H16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!I16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!J16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!K16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!L16","v":0},{"$f":"'1.Plate_layout_&HiFimix'!M16","v":0}]
[]
["Procedures",{"$nulls":9},"volume (µL/mL )"]
["1. Equilibrate AMPure XP Beads to RT for ≥30 min"]
["2. Freshly prepare 50 mL 70% ethanol (EtOH) solution (35 mL 100% EtOH + 15 mL PCR grade H2O)",{"$nulls":9},"50 mL"]
["FIRST ROUND (High MW DNA is captured on beads and discarded; library is in supernatant)"]
["3. Briefly centrifuge the plate with libraries"]
["4. Add 25 µl AMPure XP beads (vortex thoroughly before pipetting) to each well ",{"$nulls":9},"25 µL"]
["5. Seal the plate with an adhesive seal and vortex briefly till homogenous then briefly centrifuge"]
["6. Incubate at RT for 5 min"]
["7. Place on magnet and wait till liquid is clear ≥5 min"]
["8. Transfer entire supernatant (~75 µL) to new plate"]
["SECOND ROUND (Libraries are now captured by the beads while primers are cleaned away)"]
["9. Add 60 µl AMPure XP beads (vortex thoroughly before pipetting) to each well ",{"$nulls":9},"60 µL"]
["10. Seal the plate with an adhesive seal and vortex briefly till homogenous then briefly centrifuge"]
["11. Incubate at RT for 5 min"]
["12. Place on magnet and wait till liquid is clear ≥5 min"]
["13. Discard all supernatant with multichannel pipette",{"$nulls":9},"~135 µL"]
["14. WASH 2X: 1) Add 150 µL 70% EtOH, 2) incubate till clear (≥30sec), 3) discard supernatant",{"$nulls":9},"150 µL"]
["15. Remove any residual EtOH with 10 µL pipette and let air-dry for ≥ 5 min"]
["16. Remove the plate from the magnet"]
["17. Add 30 µl Low TE to each well.",{"$nulls":9},"30  µL"]
["18. Seal the plate with an adhesive seal and vortex briefly till homogenous then briefly centrifuge"]
["19. To ensure the beads are well resuspended, if necessary, you can mix by pipetting. "]
["20. Place on magnet and wait till liquid is clear ≥5 min"]
["21. Transfer 27 µl of the supernatant (containing the libraries) to a new 96w plate and seal with caps",{"$nulls":9},"27  µL"]
["SAFE STOPPING POINT (store at -15⁰C for up to 30 days)"]